Compare TLC and paper chromatography techniques.
Answer
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Hint: Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid (gas, solvent, water, etc) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which is fixed a material called the stationary phase. The different constituents of the mixture have different affinities for the stationary phase.
Complete answer:
Note:
Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for later use, and is thus a form of purification. Analytical chromatography is done normally with smaller amounts of material and is for establishing the presence or measuring the relative proportions of analytes in a mixture. The two are not mutually exclusive.
Complete answer:
| Thin Layer Chromatography (TLC) | Paper Chromatography |
| The principle of separation is adsorption. | The principle of separation is partition. |
| Less time consuming | More time consuming |
| Large amount of substance is required | Less amount of substance is required |
| In TLC, chromatographs are prepared by coating thin layers of silica gel or alumina on glass plates. | Here paper is generally used. Silica gel or alumina cannot be used because they cannot be made into sheets. |
| TLC plates can be heated in an oven for a long time. | Paper cannot be heated in an oven for a long time. |
| The different spots can be seen under the UV lamp in TLC. | Different spots cannot be seen under UV light. |
| The stationary phase of the thin layer chromatography is the glass plates coated with silica gel. | The stationary phase of paper chromatography is the water trapped in the cellulose filter paper. |
Note:
Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for later use, and is thus a form of purification. Analytical chromatography is done normally with smaller amounts of material and is for establishing the presence or measuring the relative proportions of analytes in a mixture. The two are not mutually exclusive.
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