Which of the following is not a biofertilizer?
A)Azolla- Anabaena symbiosis
B)Mycorrhiza
C)Legume- Rhizobium symbiosis
D)Algae- fungi symbiosis

Hint: The interaction between these two are widespread and facilitated the evolution of land plants and lichens. With the close physical contact, the symbiosis begins and exchange their nutrients between their cells which include carbon and nitrogen

Complete answer:
-Living microorganisms are present in biofertilizers, which colonize the rhizosphere or the interior of the plant when applied to seeds, plant surfaces, or soils. It promotes the growth of the host plant by increasing the supply or availability of primary nutrients.
-Lichens results in the symbiotic relationship between algae and fungi. Mycobiont is known as the fungal component whereas the phycobiont is known as the algal component.
-absorption of water and minerals is initiated by the fungal component and provides them to the algae.
-The algae used them with its chlorophyll to make food. That food is absorbed by the fungi.
-Lichen has an acellular structure. They are very good pollution indicators and do not grow in polluted areas.

Additional information:
Azolla- Anabaena symbiosis - A symbiotic relationship is maintained by the Azolla and the blue-green alga Anabaena. The nitrogen is provided to the fern by the Algae and the fern, in turn, provides habitat for the algae. Azolla is economically very important for the cultivation of rice because it has the property of nitrogen fixation, which makes it a very good biofertilizer.
Mycorrhiza - A symbiotic relationship is maintained by a fungus with the roots of higher plants. Water and phosphates are absorbed by the fungus from the soil and pass to the plants.
-It is healthy in solubilizing the complex organic matter into simple substances and also provides resistance to root borne pathogens.
-It increases the tolerance of a plant to salinity and drought. Hence, it is a very good biofertilizer. Glomus species are very common fungi that form a mycorrhizal association with the plants.
Legume- Rhizobium symbiosis - Symbiotic relationships maintained by the legumes with nitrogen-fixing soil bacteria called rhizobium. Their associations form the nodules on the plant root so that bacteria convert atmospheric nitrogen into ammonia that in turn used by the plants.

So, the correct answer is, “Algae- fungi symbiosis”.

Note:
-As biofertilizers, the symbiotic relationship between algae and fungi is not useful because their associations form the lichens.
-A biofertilizer is a living microorganism or dried microorganisms.
-For a long time, Rhizobium, Azotobacter, Azospirillum, mycorrhiza, and blue-green algae have been used as a biofertilizer.